University of Otago

Centre for Gene Research

Centre for Gene Research
 
University of Otago


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Centre for Gene Research

Effective Use of the GCG Suite of Programs
Sequence Analysis - 1997
This course was sponsored by the Research Theme: Gene Structure & Function

 

Mark Dalphin and Peter Stockwell again offered their course in the use of the GCG suite of sequence manipulation programs and other tools for the manipulation and interpretation of protein and DNA sequences.

Topics Covered:

  • Connecting your PC or Macintosh to the GCG programs
  • "Making pretty pictures" or the use of GCG Graphics
  • Entering and Editing Sequences
  • Retrieving Sequences from the Databases
  • Blast and FASTA searches - which do you use and why
  • Sequence alignment and why FASTA is unsuitable
  • Restriction enzyme mapping
  • Multiple sequence alignment and editing
  • Translation of DNA sequence into peptide sequence
  • Back translation of proteins into DNA
  • Design of PCR primers

Coverage included supplementary, non-GCG programs included several on the Internet:

  • Manipulation of DNA Sequence from the ABI Sequencer
  • Transferring sequences from your computer to GCG programs
  • Retrieving sequences via the World Wide Web
  • The multiple sequence editor, HOMED, and why to use it.

Specifics: where the course was held:

Date: Monday 23 June - Friday 27 June
Time: Mornings, 9 AM - 1 PM
Where: North CAL Lab, in the Science Library foyer

Cost: $100

Contact: Mark Dalphin, Rm 111b Biochemistry, 3-479-7841
E-mail: mdalphin@sanger.otago.ac.nz

Course numbers were limited to 22 participants -contact Mark Dalphin for more information. Some financial assistance was available for students who required it. There are a few Course Manuals available @ $10.00 for who would like an extra manual.

 

Centre for Gene Research
Centre for Gene Research
Centre for Gene Research

 

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Centre for Gene Research
Centre for Gene Research
Centre for Gene Research
Centre for Gene Research