Fluorogenic 5' nuclease assay(TaqMan_ chemistry)

forward primer

reverse primer

3'

3'

5'

5'

3'

5'

5'

1. Polymerisation

probe

3'

3'

5'

5'

3'

5'

5'

2. Strand displacement

3'

3'

5'

5'

3'

5'

5'

3. Cleavage

3'

5'

5'

3'

5'

5'

4. Polymerisation completed

R = Reporter

Q = Quencher

3'

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